A-Chip consists of 2 wells connected by a microchannel. iPSC-derived neuronal spheres are seeded into the smaller well and axons grow into the microchannel. After culturing for several weeks, the axons will self-organize into a bundle and extend into the far well. Cell body and axon bundle can be isolated separately for protein analysis, RNA extraction, and other molecular analyses.
| Chip materials | COP (cyclic olefin polymer; Zeon Corporation, Japan) Transparent, chemical resistant, heat resistant, low protein adsorption, low elution（US Pharmacopeia Class Ⅵ compliant） |
|---|---|
| Outer dimensions | 38 mm(W) x 10 mm(D) x 9 mm(H) |
| Microchannel length | 18.5 mm |
| Selling unit | 8 chips/plate |
B-Chip consists of 2 wells connected by a microchannel. The 2 wells are separated by a wall, allowing researchers to culture cells in different media in each well. iPSC-derived neuronal spheres are seeded into the smaller well and axons grow into the microchannel. During culture, axons self-organize into a bundle and extend into the far well where they branch out and expand uniformly into growth cones. B-Chips can be used to co-culture target cell types and to induce human synapse formation using our synaptogenic beads. B-chip fits 48 chips into an SBS standard 96-well plate, making it compatible with imaging systems.
| Chip materials | COP (cyclic olefin polymer; Zeon Corporation, Japan) Transparent, chemical resistant, heat resistant, low protein adsorption, low elution（US Pharmacopeia Class Ⅵ compliant） |
|---|---|
| Outer dimensions | 17.9 mm(W) x 8.8 mm(D) x 12.4 mm(H) |
| Microchannel length | 2.6 mm |
| Selling unit | 48 chips/plate (96 well plate format) |
P-Chip is our product for academic research, although we sell to non-academic researchers, as well. This product consists of 8 PDMS inseparable chips mounted on a glass slide. Each chip consists of 2 wells connected by a microchannel. Neuronal cells of interest can be seeded into the smaller well to induce axonal growth into the microchannel. After culturing for several weeks, the axons will self-organize into a bundle and extend into the far well.
| Chip materials | PDMS-glass (Polydimethylsiloxane-glass) |
|---|---|
| Outer dimensions | 4.0 cm(W) x 1.8 cm(D) x 1.0 mm(H) |
| Microchannel length | 9.0 mm |
| Selling unit | 8 chips/plate or individual chips |
| A-Chip/ P-Chip | B-Chip | Synaptogenic Beads | |
|---|---|---|---|
| Immunostaining | ○ | ||
| Calcium imaging | ○ | ○ | |
| DNA/RNA extraction | ○ | ||
| Western Blotting | ○ | ||
| Neurotransmitter quantification | ○ | ||
| Co-culture with other cell types | ○ | ||
| Toxicity testing | ○ | ○ | |
| Axon growth comparison | ○ | ||
| TEM/SEM images (Nerve Organoid™) | ○ | ||
| SEM images (synapse formation) | ○ | ||
| High-throughput screening | ○ |
| Product number | Product name | Specifications |
|---|---|---|
| JKSK_S0001A | A-Chip | 8 chips/plate or individually |
| JKSK_S0001B | B-Chip | 48 chips/plate or individually |
| JKSK_S0001U | P-Chip | 8 chips/plate |
To place an order, please contact us at: info@jiksak.co.jp
