Our patented Nerve Organoid™ consists of 3D nerve tissue in a unique microfluidics device. The 3D nerve tissue is derived from human iPS nerve cells. It closely resembles an in vivo nerve with a cell body and axons that self-organize into bundles as they extend through the device’s microchannel.
＊Patent #6430680 – “Apparatus for culturing nerve cells, method for culturing nerve cells, cultured nerve cells, method for analyzing and identifying proteins in axon bundles, and method for using nerve cells”（Patented in Japan and the USA）
Video shot at 10X magnification and has been sped up by approximately 17x
Jiksak’s Nerve Organoid™ accelerates drug development for neurodegenerative diseases by reducing the number of unsuitable compounds from advancing to animal or clinical trials. Since the iPS cell-derived 3D nerve tissue shares many biological characteristics with in vivo human neural tissue, researchers can predict the safety, efficacy, and toxicity of drug candidates swiftly and accurately at the preclinical stage. A Nerve Organoid™ has separate compartments for the 3D cell body and axon bundle, which can be separated in a one-step process. This allows researchers to perform evaluations not feasible with conventional 2D neuronal cultures.
Jiksak Bioengineering sells proprietary culture chip devices for the culture of Nerve OrganoidTM . Each chip is made up of a microfluidic channel fabricated using microfabrication technology and is sandwich by culture wells at both ends. When culturing neuron spheroid cell body in one well, axons from the cell body is capable of extending into the channel to form self-organized axon bundles.
A-chip is a device that can fabricate long axon bundles (about 2 cm). After culturing for several weeks, the axons will self-organize into a bundle and extend into the far well. Cell body and axon bundle can be isolated separately for protein analysis, RNA extraction, and other molecular analyses.
| Chip materials | COP (cyclic olefin polymer; Zeon Corporation, Japan) Transparent, chemical resistant, heat resistant, low protein adsorption, low elution（US Pharmacopeia Class Ⅵ compliant） |
|---|---|
| Outer dimensions | 38 mm(W) x 10 mm(D) x 9 mm(H) |
| Microchannel length | 18.5 mm |
| Selling unit | Available individually |
B-Chip consists of 2 wells connected by a microchannel. The 2 wells are separated by a wall, allowing researchers to culture cells in different media in each well. iPSC-derived neuronal spheres are seeded into the smaller well and axons grow into the microchannel. During culture, axons self-organize into a bundle and extend into the far well where they branch out and expand uniformly into growth cones. B-Chips can be used to co-culture target cell types and to induce human synapse formation using our synaptogenic beads. B-chip fits 48 chips into an SBS standard 96-well plate, making it compatible with imaging systems.
| Chip materials | COP (cyclic olefin polymer; Zeon Corporation, Japan) Transparent, chemical resistant, heat resistant, low protein adsorption, low elution（US Pharmacopeia Class Ⅵ compliant） |
|---|---|
| Outer dimensions | 17.9 mm(W) x 8.8 mm(D) x 12.4 mm(H) |
| Microchannel length | 2.6 mm |
| Selling unit | Available individually |
This product consists of 8 PDMS inseparable chips mounted on a glass slide. Each chip consists of 2 wells connected by a microchannel. Neuronal cells of interest can be seeded into the smaller well to induce axonal growth into the microchannel. After culturing for several weeks, the axons will self-organize into a bundle and extend into the far well.
| Chip materials | PDMS-glass (Polydimethylsiloxane-glass) |
|---|---|
| Outer dimensions | 4.0 cm(W) x 1.8 cm(D) x 1.0 mm(H) |
| Microchannel length | 9.0 mm |
| Selling unit | Available individually |
| A-Chip/ P-Chip | B-Chip | Synaptogenic Beads | |
|---|---|---|---|
| Immunostaining | ○ | ||
| Calcium imaging | ○ | ○ | |
| DNA/RNA extraction | ○ | ||
| Western Blotting | ○ | ||
| Neurotransmitter quantification | ○ | ||
| Co-culture with other cell types | ○ | ||
| Toxicity testing | ○ | ○ | |
| Axon growth comparison | ○ | ||
| TEM/SEM images (Nerve Organoid™) | ○ | ||
| SEM images (synapse formation) | ○ | ||
| High-throughput screening | ○ |
| Product number | Product name | Specifications |
|---|---|---|
| JKSK_S0001A | Nerve Organoid™ A-chip（cop/cop） | 8 chips/plate or individually |
| JKSK_S0002A | Nerve Organoid™ A-chip（PDMS/Glass） | 8 chips/plate or individually |
| JKSK_S0001B | Nerve Organoid™ B-chip | 48 chips/plate or individually |
| JKSK_S0001U | Nerve Organoid™ P-chip | 8 chips/plate |